By bus
Greyhound
Greyhound busses provide travel throughout the state, along all major cities and towns along I-43, US 45, I-90, I-94, and more, servicing among others Appleton, Brookfield, Eau Claire, Fond du lac, Green Bay, Kenosha, La Crosse, Milwaukee, Oshkosh, Racine, Stevens Point, Waukesha, Wisconsin Dells, and more. They also sell tickets often cheaper for other companies.
Indian Trails
Daily service from Chicago via Milwaukee to Michigan's Upper Peninsula stopping in Marinette, Green Bay, Manitowoc, Sheboygan. Onward connections are available. Buses have wifi and power outlets.
Jefferson Lines
Connects Milwaukee, Madison, and La Crosse daily with interim stops across Wisconsin. Jefferson prides itself on its 'eco-friendly' new coaches. Connections are available at Minneapolis.
Wisconsin Coach
Wisconsin Coach offers several services around Southeast Wisconsin to/from Milwaukee.
Train
Amtrak has two lines that service the state. The Hiawatha has 7 daily roundtrips between Milwaukee and Chicago, with additional stops outside of Racine and at Mitchell Field Airport. The Empire Builder runs once daily, and effectively parallels I-94 to Chicago coming all the way from Seattle, Washington. The Train station has recently been remodeled into a nice clean and modern looking building located downtown.
General
Getting Around Wisconsin Without A Car: A Public Transportation Guide pdf (http://www.dot.wisconsin....).
By plane
Wisconsin has two international airports, Mitchell International in Milwaukee MKE, which is a hub for Midwest Airlines/Frontier Airlines, and Austin Straubel International in Green Bay GRB. Regional airports with scheduled service exist in Madison MSN, Appleton ATW, Wausau/Stevens Point CWA, Rhinelander RHI, La Crosse LSE, and Eau Claire EAU. Service to the far western "Indianhead" region of the state can be found across the Minnesota border in Minneapolis MSP and Duluth DLH. Travel by air within Wisconsin has become rather impractical in the last 25 years. Unless traveling to/from Milwaukee, travel between Wisconsin cities by air requires a connection in Milwaukee, Chicago, Minneapolis or even Detroit. It is usually faster and less expensive to travel within the state via automobile.